Neutral compounds, such as patulin, or mixtures of neutral and charged compounds can be analyzed by MECC . There are various advantages for using MECC. Only a small amount (2 ml) of sample is used and less organic solvent is consumed compared to HPLC methods. The method is rapid, with total run time of 10 minutes, and has a low limit of detection (3.8 mg patulin/l). For samples having patulin levels < 75 mg/l, 2 ml of sample is extracted with ethyl acetate. The extract is passed through anhydrous sodium sulfate, evaporated under nitrogen, reconstituted in 0.1 ml acidic water solution (pH 4), and analyzed immediately by MECC.
Was this article helpful?