Fresh wounds feature an immediate release of fluid from ruptured vacuoles and plasmalemma. This "cell sap'' congests the intercellular spaces in and beneath the damaged cells creating instant fluid channels . Within seconds of contact, particulate matter or aqueous suspensions may be transported up to 1 cm laterally from a puncture wound in a leaf . This concept of rapid internalization in wounds is supported by tests on the disinfection of wounds on tomato fruit. Bartz et al.  observed that within 5 seconds of application of an aqueous cell suspension of E. carotovora subsp. carotovora to the flat surface of a fresh wound on a tomato fruit, a portion of that population could not be completely eliminated when the fruit was washed for 2 minutes in 100 ppm free chlorine at pH 7.0 in a scale model flume. Gently rubbing the submerged wound surface with a soft bristle brush or with a gloved finger did not improve disinfection efficacy. In contrast, 10 ppm free chlorine present over similar wounds on fruit in the same flume prevented inoculation by a similar suspension, whereas just 5 ppm prevented most wounds from becoming inoculated. A water-soluble dye could be completely rinsed from these wound surfaces if the fruit was rinsed under running tap water within 6 seconds of dye application. If the wash was delayed more than 6 seconds a portion of the dye could be observed embedded in intercellular spaces beneath the wound.
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