Internalization in Wounds

Fresh wounds feature an immediate release of fluid from ruptured vacuoles and plasmalemma. This "cell sap'' congests the intercellular spaces in and beneath the damaged cells creating instant fluid channels [25]. Within seconds of contact, particulate matter or aqueous suspensions may be transported up to 1 cm laterally from a puncture wound in a leaf [43]. This concept of rapid internalization in wounds is supported by tests on the disinfection of wounds on tomato fruit. Bartz et al. [50] observed that within 5 seconds of application of an aqueous cell suspension of E. carotovora subsp. carotovora to the flat surface of a fresh wound on a tomato fruit, a portion of that population could not be completely eliminated when the fruit was washed for 2 minutes in 100 ppm free chlorine at pH 7.0 in a scale model flume. Gently rubbing the submerged wound surface with a soft bristle brush or with a gloved finger did not improve disinfection efficacy. In contrast, 10 ppm free chlorine present over similar wounds on fruit in the same flume prevented inoculation by a similar suspension, whereas just 5 ppm prevented most wounds from becoming inoculated. A water-soluble dye could be completely rinsed from these wound surfaces if the fruit was rinsed under running tap water within 6 seconds of dye application. If the wash was delayed more than 6 seconds a portion of the dye could be observed embedded in intercellular spaces beneath the wound.

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