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figure 26.9 Backscattered electron image of S. enterica serovar Thompson cells on a cilantro leaf after their inoculation onto cilantro plants. Gold-labeled flagellar antibodies that are specific to this serovar are shown binding to the flagellum, which appears to be anchored to the plant surface (arrows). Bright dots are 10 nm gold particles. Bar, 500 nm. (Micrograph courtesy of Delilah F. Wood.)

figure 26.9 Backscattered electron image of S. enterica serovar Thompson cells on a cilantro leaf after their inoculation onto cilantro plants. Gold-labeled flagellar antibodies that are specific to this serovar are shown binding to the flagellum, which appears to be anchored to the plant surface (arrows). Bright dots are 10 nm gold particles. Bar, 500 nm. (Micrograph courtesy of Delilah F. Wood.)

labeling in EM based on the binding affinity of lectins, enzymes, or proteins to specific molecules. For a discussion and description of methodology regarding the use of gold labeling and EM to investigate plant-microbe interactions, the reader is referred to an excellent review by Benhamou and Belanger [70].

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