Enumeration

Enumeration of the cell population may provide important information related to spoiled packaged products having obvious medicinal off-aroma. Basic plating techniques for enumeration are spread plating, including spiral plates and pour plating [59,60]. In the case of clear liquids, a filtration technique may be used to concentrate cells from a large sample volume, which increases test sensitivity. For liquids containing particulate matter, such as cloudy juices, a most probable number (MPN) technique is appropriate [60]. Enumeration by the MPN technique would be conducted using an appropriate broth medium such as filter-sterilized juice, or a low pH minimal nutrient broth such as Ali Broth [44] or BAT Broth [45,54].

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