Ultimately, of course, it is essential to determine functionality of the overexpressed transcript. These analyses can take the form of overexpressing the encoded protein of interest, either transiently or stably in cell lines or by the generation of transgenic mice. The advent of siRNA makes it substantially more accessible, without necessarily having to generate knockout mice in which the gene of interest has been removed, to assess the effect of eliminating the expression of the transcript in question. Whether overexpression or knockout technology is used, it is necessary to possess a sufficiently robust assay to assess the effect of modifying expression of the protein of interest. Such assays might range from a global mRNA expression array analysis of mutant and wild-type cells to a promoter- or polymerase chain reaction-based assay to specifically assess the role of a protein in a specific cellular outcome of interest.
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This ebook provides an introductory explanation of the workings of the human body, with an effort to draw connections between the body systems and explain their interdependencies. A framework for the book is homeostasis and how the body maintains balance within each system. This is intended as a first introduction to physiology for a college-level course.