Day Fetal Mouse Lung 15Day Fetal Mouse Lung

Branching Angiogenesis Connection of Central and Peripheral Systems

Figure 1 Functional heterogeneity along the lung vascular tree can be resolved by segment-specific responses to agonists such as thapsigargin and is related to the unique differentiation of endothelial cells during their normal embryological development. (A) Thapsigargin induces intercellular gaps in artery/arteriole and venule/vein segments, but not in capillary segments. For experimental details see Ref. [5]. (B) Scanning electron microscopy evaluation of vascular casting reveals an incomplete pulmonary circulation in the early pseudoglan-dular phase of lung development (left panel), even though blood islands can be resolved in parallel by transmission electron microscopy (not shown). Near the end of the pseudoglandular phase of lung development, capillaries can be resolved intact with the pulmonary artery (right panel). For experimental details see Ref. [6]. (C) Rat pulmonary macro- and microvascular endothelial cells can be distinctly resolved in the intact circulation by their interaction with Griffonia simplicifolia and Helixpomatia (toppanel). (see color insert) Since lectins are agglutinins, cells isolated from each segment agglutinate with exposure to lectin even in the presence of trypsin (bottom panel). For experimental details see Ref. [7].

Figure 1 Functional heterogeneity along the lung vascular tree can be resolved by segment-specific responses to agonists such as thapsigargin and is related to the unique differentiation of endothelial cells during their normal embryological development. (A) Thapsigargin induces intercellular gaps in artery/arteriole and venule/vein segments, but not in capillary segments. For experimental details see Ref. [5]. (B) Scanning electron microscopy evaluation of vascular casting reveals an incomplete pulmonary circulation in the early pseudoglan-dular phase of lung development (left panel), even though blood islands can be resolved in parallel by transmission electron microscopy (not shown). Near the end of the pseudoglandular phase of lung development, capillaries can be resolved intact with the pulmonary artery (right panel). For experimental details see Ref. [6]. (C) Rat pulmonary macro- and microvascular endothelial cells can be distinctly resolved in the intact circulation by their interaction with Griffonia simplicifolia and Helixpomatia (toppanel). (see color insert) Since lectins are agglutinins, cells isolated from each segment agglutinate with exposure to lectin even in the presence of trypsin (bottom panel). For experimental details see Ref. [7].

Essentials of Human Physiology

Essentials of Human Physiology

This ebook provides an introductory explanation of the workings of the human body, with an effort to draw connections between the body systems and explain their interdependencies. A framework for the book is homeostasis and how the body maintains balance within each system. This is intended as a first introduction to physiology for a college-level course.

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