Assays of Enzyme Activity

Most PQQ- and FAD-dependent dehydrogenases can be assayed using artificial electron acceptors such as potassium ferricyanide or phenazine methosulfate (PMS) [3]. In the case of potassium ferricyanide, enzyme activity can be assayed over a broad pH range, from highly acidic to highly alkaline conditions. On the other hand, enzyme activity measurement with PMS combined with dichlorophe-nol indophenol (DCIP) is invalid at acidic pH below 6 due to non-enzymatic decolorization of the electron acceptor used. Thus, the assay with PMS-DCIP is valid in the neutral to alkaline regions.

It is also worth noting that when enzymes containing a heme c component in the enzyme molecule or membrane fraction are used, the enzyme activities can be easily assayed with potassium ferricyanide. However, enzyme activity measurement with PMS-DCIP is invalid if the enzymes do not contain the heme c component after solubilization from the membrane.

6 | 1 Biooxidation with PQQ- and FAD-Dependent Dehydrogenases 1.3

Heal Yourself With Qi Gong

Heal Yourself With Qi Gong

Qigong also spelled Ch'i Kung is a potent system of healing and energy medicine from China. It's the art and science of utilizing breathing methods, gentle movement, and meditation to clean, fortify, and circulate the life energy qi.

Get My Free Ebook

Post a comment