Modular Approach to Biotransformation Using Microbial Cytochrome P450 Monooxygenases 177

Akira Arisawa and Hitosi Agematu

7.1 Introduction 177

7.2 Experimental Outline 180

7.2.1 Gene Sequences 180 pT7NS-camAB 180 Plasmids to Express Bacterial CYPs 180

7.2.2 Preparation of Whole Cell Catalysts 181

7.2.3 Biotransformation of the CYP Substrates 181 Carbomycin A 181 Pravastatin 182 7-Hydroxycoumarin 182

7.2.4 Biotransformation by CYP Reaction Array 182

7.3 Bacterial CYP Expression System in E. coli 183

7.4 Construction of a Bacterial CYP Library 185

7.5 Construction of a Bacterial CYP Reaction Array 186

7.6 Application of the CYP Reaction Array to Biotransformation Screening 187

References 190

Heal Yourself With Qi Gong

Heal Yourself With Qi Gong

Qigong also spelled Ch'i Kung is a potent system of healing and energy medicine from China. It's the art and science of utilizing breathing methods, gentle movement, and meditation to clean, fortify, and circulate the life energy qi.

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