Agarose Gel Electrophoresis of PsfIDigested pKN800 Class

Use an 0.8 agarose gel to separate the restriction fragments in your DNA samples. This percentage of agarose will separate linear DNA molecules that range from 0.6 to 10 kb. a. Seal the edges of a clean, dry, plastic gel-casting tray supplied with the electrophoresis apparatus. Our apparatus comes with a sealing clamp, but some models require that the ends be sealed with tape. Install the well former (comb) on the casting tray set the comb depth so that a microscope slide...

Highly Recommended Resources for Writers

Scientific English A Guide for Scientists and Other Professionals. Phoenix, AZ Oryx Press. ISBN 0-89774-722-4. Scientific English is an excellent companion for How to Write and Publish a Scientific Paper. In this book, Day discusses parts of speech, grammar, phrases, clauses, sentences, and paragraphs. It is not as tedious as it sounds due to Day's straightforward, readable prose. Most students will benefit from reading this book. Kanare, Howard M. (1985). Writing the...

Plasmid DNA Preparation

Insertional Inactivation Pbr322

In this course, you will learn to work with plasmids because you will probably use them often in your career. Many methods have been developed to purify plasmid DNA from E. coli and other bacteria. These procedures employ a variety of methods to lyse cells and separate plasmid DNA from other cellular components. Detergents, organic solvents, alkali, or heat can lyse bacteria. Three factors dictate which method to use the size of the plasmid, the bacterial strain, and the technique used to...