An immediate cellmaterial relationship model Cuprophan Swiss 3T3 murine fibroblast cell line

3.2.1 A cellulose substratum: Cuprophan

3.2.1.1 Nature

Cuprophan (CU), a regenerated cellulose membrane produced by the cuprammonium process, appears to be very hydrophilic (due to OH groups in the cellobiose units of the cellulose molecule) as attested by a 22° water contact angle.

We compared its ability to adsorb serum proteins of the culture medium to the moderately wettable acrylonitrile AN69 membrane (40° contact angle). Those biomaterials are both currently used in bioreactors and hemodialyzers.

3.2.1.2 In vitro serum protein adsorption

Low ability of CU to adsorb serum adhesive proteins

Immunoblot analysis of FN and VN adsorbed on 45 min FBS-conditioned AN69 and CU was performed. FN was not detected in the eluate of CU but was resolved into two bands of 230 and 220 kDa in the eluate of AN69. VN was resolved into a faint band of 74 kDa in the eluate of CU and a very large VN band was detected in the sample from AN69. CU adsorbed 60-fold less serum proteins than AN69 in our experimental system [4].

Low binding strength ofadsorbed total serum proteins to CU

SDS PAGE protein profiles of supernatants (250,230, 170, 130, 110, 75,66, 58, and 52 kDa) obtained from washes of 45 min FBS-conditioned AN69 and CU were analyzed. Proteins were detected in all washes of FBS-conditioned AN69 with 10%, 30%, 50%, and 70% isopropanol. In contrast, FBS proteins were completely removed from FBS-conditioned CU by the 10% and 30% isopropanol washes, attesting a weak strength of serum adsorbed proteins on CU [4].

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