Longterm behavior of murine melanoma B16 F10 explants on CU

B16 F10 murine melanoma cells possess a highly metastatic potency when injected to mice. Tumor explants (1-2 mm3) layered on semi-solid culture medium were covered with 0.7 cm2 CU or AN69 pieces and cultured for 14 days. Proliferation, adhesion, and migration of sorting cells were assessed from the cell layer developed around each explant. Surface area and cell counts after trypsin-EDTA detachment kinetics can give thus a good evaluation of cell behavior 31 . Proliferation and migration of...

Historical characteristics of wound dressing fibers and wound healing

Through the ages, both vegetable and animal fibers have been applied to human wounds to stop bleeding, absorb exudate, alleviate pain and provide a protective barrier for the formation of new tissue. Some milestones of wound dressing development down through the ages are summarized in Figure 2.1. Early humankind employed many different materials from the natural surroundings including resin-treated cloth, leaves, and wool-based materials with a variety of substances including eggs and honey....

The origins of moist wound dressings and the ideal wound dressing

The concept that wounds heal best when kept dry was chiefly espoused in wound management up until the late fifties because it was thought that bacterial infection couldbest be prevented by absorbing and removing all wound exudate. Consequently, most wounds were treated with cotton or viscose fiber material under dry conditions. However, in the early sixties Winter 6 and Hinman and Maiback 7 showed that the rate of reepithelialization increases in a moist wound versus a wound kept dry. Occlusion...