Ackermann M, Padmanabhan R 2001 De novo synthesis of RNA by the dengue virus RNA-dependent RNA polymerase exhibits temperature dependence at the initiation but not elongation phase. J Biol Chem 276:39926-39937 Alvarez DE, Lodeiro MF, Luduena SJ, Pietrasanta LI, Gamarnik AV 2005 Long-range RNA-

RNA interactions circularize the dengue virus genome. J Virol 79:6631-6643 Arias CF, Preugschat F, Strauss JH 1993 Dengue 2 virus NS2B and NS3 form a stable complex that can cleave NS3 within the helicase domain. Virology 193:888-899 Bartelma G, Padmanabhan R 2002 Expression, purification, and characterization of the RNA 5'-triphosphatase activity of dengue virus type 2 nonstructural protein 3. Virology 299:122-132

Benarroch D, Selisko B, Locatelli GA et al 2004 The RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activities of Dengue virus protein NS3 are Mg2+-dependent and require a functional Walker B motif in the helicase catalytic core. Virology 328:208-218

Chambers TJ, Grakoui A, Rice CM 1991 Processing of the yellow fever virus nonstructural polyprotein: a catalytically active NS3 proteinase domain and NS2B are required for cleavages at dibasic sites. J Virol 65:6042-6050 Chambers TJ, Nestorowicz A, Amberg SM, Rice CM 1993 Mutagenesis of the yellow fever virus NS2B protein: effects on proteolytic processing, NS2B-NS3 complex formation, and viral replication. J Virol 67:6797-6807 Chanprapaph S, Saparpakorn P, Sangma C et al 2005 Competitive inhibition of the dengue virus NS3 serine protease by synthetic peptides representing polyprotein cleavage sites. Biochem Biophys Res Commun 330:1237-1246 Chu PW, Westaway EG 1987 Characterization of Kunjin virus RNA-dependent RNA polymerase: reinitiation of synthesis in vitro. Virology 157:330-337 Clum S, Ebner KE, Padmanabhan R 1997 Cotranslational membrane insertion of dengue virus type 2 NS2B/NS3 serine proteinase precursor is required for efficient processing in vitro. J Biol Chem 272:30715-30723

Corver J, Lenches E, Smith K et al 2003 Fine mapping of a cis-acting sequence element in yellow fever virus RNA that is required for RNA replication and cyclization. J Virol 77:2265-2270

Cui T, Sugrue RJ, Xu Q et al 1998 Recombinant dengue virus type 1 NS3 protein exhibits specific viral RNA binding and NTPase activity regulated by the NS5 protein. Virology 246:409-417

Egloff MP, Benarroch D, Selisko B, Romette JL, Canard B 2002 An RNA cap (nucleoside-2'-O-)-methyltransferase in the flavivirus RNA polymerase NS5: crystal structure and functional characterization. EMBO J 21:2757-2768 Falgout B, Pethel M, Zhang YM, Lai CJ 1991 Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins. J Virol 65:2467-2475

Falgout B, Miller RH, Lai C-J 1993 Deletion analysis of dengue virus type 4 nonstructural protein NS2B: Identification of a domain required for NS2B-NS3 protease activity. J Virol 67:2034-2042

Ganesh VK, Muller N, Judge K et al 2005 Identification and characterization of nonsubstrate based inhibitors of the essential dengue and West Nile virus proteases. Bioorg Med Chem 13:257-264

Grun JB, Brinton MA 1986 Characterization of West Nile virus RNA-dependent RNA polymerase and cellular terminal adenylyl and uridylyl transferases in cell-free extracts.

J Virol 60:1113-1124

Kapoor M, Zhang L, Ramachandra M et al 1995 Association between NS3 and NS5 proteins of dengue virus type 2 in the putative RNA replicase is linked to differential phosphorylation of NS5. J Biol Chem 270:19100-1916 Khromykh AA, Meka H, Guyatt KJ, Westaway EG 2001 Essential role of cyclization sequences in flavivirus RNA replication. J Virol 75:6719-6728 Leung D, Schroder K, White H et al 2001 Activity of recombinant dengue 2 virus NS3 protease in the presence of a truncated NS2B co-factor, small peptide substrates, and inhibitors. J Biol Chem 276:45762-45771 Li H, Clum S, You S, Ebner KE, Padmanabhan R 1999 The serine protease and the RNA-stimulated NTPase domains of dengue virus type 2 converge within a region of 20 amino acids. J Virol 73:3108-3116 Li J, Lim SP, Beer D et al 2005 Functional profiling of recombinant NS3 proteases from all four serotypes of dengue virus using tetrapeptide and octapeptide substrate libraries. J Biol Chem 280:28766-28774 Lindenbach BD, Rice CM 2003 Molecular biology of flaviviruses. Adv Virus Res 59:23-61 Lo MK, Tilgner M, Bernard KA, Shi PY 2003 Functional analysis of mosquito-borne flavivirus conserved sequence elements within 3' untranslated region of West Nile virus by use of a reporting replicon that differentiates between viral translation and RNA replication. J Virol 77:10004-10014

Murthy HM, Clum S, Padmanabhan R 1999 Dengue virus NS3 serine protease. Crystal structure and insights into interaction of the active site with substrates by molecular modeling and structural analysis of mutational effects. J Biol Chem 274:5573-5580 Murthy HM, Judge K, DeLucas L, Padmanabhan R 2000 Crystal structure of Dengue virus NS3 protease in complex with a Bowman-Birk inhibitor: implications for flaviviral polyprotein processing and drug design. J Mol Biol 301:759-767 Nomaguchi M, Ackermann M, Yon C, You S, Padmanbhan R 2003a De novo synthesis of negative-strand RNA by dengue virus RNA-dependent RNA polymerase in vitro: nucleotide, primer, and template parameters. J Virol 77:8831-8842 Nomaguchi M, Teramoto T, Yu L, Markoff L, Padmanabhan R 2003b Requirements for West Nile virus (—)- and (+)-strand subgenomic RNA synthesis in vitro by the viral RNA-depend-ent RNA polymerase expressed in Escherichia coli. J Biol Chem 279:12141-12151

Uchil PD, Satchidanandam V 2003a Architecture of the flaviviral replication complex: protease, nuclease and detergents reveal encasement within double-layered membrane compartments. J Biol Chem 278:24388-24398 Uchil PD, Satchidanandam V 2003b Characterization of RNA synthesis, replication mechanism, and in vitro RNA-dependent RNA polymerase activity of Japanese encephalitis virus. Virology 307:358-371

Utama A, Shimizu H, Morikawa S et al 2000 Identification and characterization of the RNA

helicase activity of Japanese encephalitis virus NS3 protein. FEBS Lett 465:74-78 Valle RP, Falgout B 1998 Mutagenesis of the NS3 protease of dengue virus type 2. J Virol 72:624-632

Wengler G, Czaya G, Farber PM, Hegemann JH 1991 In vitro synthesis of West Nile virus proteins indicates that the amino-terminal segment of the NS3 protein contains the active centre of the protease which cleaves the viral polyprotein after multiple basic amino acids. J Gen Virol 72:851-858 Yon C, Teramoto T, Mueller N et al 2005 Modulation of the nucleoside triphosphatase/RNA helicase and 5'-RNA triphosphatase activities of dengue virus type 2 nonstructural protein 3 (NS3) by interaction with NS5, the RNA-dependent RNA polymerase. J Biol Chem 280:27412-27419

You S, Padmanabhan R 1999 A novel in vitro replication system for Dengue virus. Initiation of RNA synthesis at the 3'-end of exogenous viral RNA templates requires 5'-and 3'-terminal complementary sequence motifs of the viral RNA. J Biol Chem 274:33714-33722

You S, Falgout B, Markoff L, Padmanabhan R 2001 In vitro RNA synthesis from exogenous dengue viral RNA templates requires long range interactions between 5'- and 3'-terminal regions that influence RNA structure. J Biol Chem 276:15581-15591 Yusof R, Clum S, Wetzel M, Murthy HM, Padmanabhan R 2000 Purified NS2B/NS3 serine protease of dengue virus type 2 exhibits cofactor NS2B dependence for cleavage of substrates with dibasic amino acids in vitro. J Biol Chem 275:9963-9969 Zhang L, Mohan PM, Padmanabhan R 1992 Processing and localization of Dengue virus type 2 polyprotein precursor NS3-NS4A-NS4B-NS5. J Virol 66:7549-7554

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