Analysis of regions of the 5 and 3 UTRs involved in DENV translation and RNA synthesis

Denv Utr Structure

Viral translation and RNA synthesis of positive-sense RNA viruses is often regulated by the 5' and 3' UTRs. Previously, we had demonstrated a role for the DENV-2 terminal 3'SL in stimulating viral translation via deletion analysis in RNA reporter constructs (Holden & Harris 2004). To examine other regions of the DENV-2 UTRs, we targeted conserved domains in the 5' and 3' UTRs by complementary peptide-conjugated phosphorodiamidate morpholino oligomers (P-PMOs), as depicted in Fig. 3. The...