Heat again to repeat the process.

Each double-stranded DNA is identical to original DNA.

Restriction enzymes

Cutting DNA: Restriction Enzymes

To cut long DNA molecules into shorter pieces, biologists use bacterial proteins called restriction enzymes. Restriction enzymes recognize specific short DNA sequences, and cut the DNA in or near the sequence, as Figure 13-2 shows. Some restriction enzymes leave DNA overhangs that function like "sticky ends" so that other pieces of DNA with complementary sequences can bind to them.

Vector DNA


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