Part D

Analyzing a Gel

21. To stain a gel, carefully place the gel (wells up) into a staining tray. Pour WARD'S DNA stain into the staining tray until the gel is completely covered. Cover the staining tray, and label it with your initials. Allow the stain to sit for at least 2 hours. Next, carefully pour the stain into the sink drain, and flush it down the drain with water. Do not let the stained gel slip out of the staining tray.

22. To destain a gel, cover the gel with distilled water by pouring water to one side of the gel. Let the gel sit overnight (or at least 8-12 hours). The bands of DNA will appear as purple lines against a light background.

23. Calculate the Rf for each fragment using the following equation:

distance inmmthatDNAfragmentmigrated distanceinmm fromwelltothe dye

24. 4Êh Dispose of your materials according to ^^ your teacher's directions, and wash your hands before leaving the lab.

Analysis and Conclusions

1. Which two samples appear to have the same pattern of DNA bands?

2. Which restriction enzyme cut the DNA in the unknown sample? Justify your answer.

3. What are some measures that you took to prevent contamination of your DNA samples during this lab?

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