Part D

Preparing a Wet Mount of Rhizobium nodules on your bean plant's roots. Draw a root with a nodule in your lab report, and label each structure. ^^CAUTION Use the scalpel with care. A

▼Scalpel is a very sharp instrument. When cutting, make sure that the blade faces away from your body. If you cut yourself, quickly apply direct pressure to the wound, and call for your teacher. Remove a large nodule from the bean root, and carefully cut the nodule in half with a scalpel. The pink nodules contain active nitrogen-fixing bacteria. The green nodules contain bacteria but cannot fix nitrogen because they are actively reproducing. Rhizobium bacteria will begin fixing nitrogen only after they stop reproducing. View the cross section under a stereoscope. Note the arrangement of symbiotic cells within the nodule. Draw and label this arrangement.

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