Part C

Observing Prepared Slides of Bone

21. /ij\ CAUTION Do not use electrical equipment ^ near water or with wet hands or clothing.

Using a compound light microscope, focus on a prepared slide of bone by using low power, and then switch to high power. Locate a Haversian canal, the darkly stained circle in the center of a set of lamellae. Find the darkly stained osteocytes between the lamellae.

22. In your lab report, draw and label the following bone structures: Haversian canal, lamella, and osteocyte.

Analysis and Conclusions

1. What effect did water loss have on the bone? What effect did mineral loss have on the bone?

2. Why did you have to dehydrate the bone before measuring its mass in Part B?

3. What percentage of bone is water? What percentage of bone is made of minerals?

4. If you were to prepare a slide using the dehydrated and demineralized bone, what do you think the bone would look like?

5. What happened when the demineralized bone was dried? Why do you think this happened?

6. If a person's diet lacked calcium, how could this affect his or her bones?

Further Inquiry

Research the differences in the amount and distribution of different bone types from various parts of the human skeleton.

Essentials of Human Physiology

Essentials of Human Physiology

This ebook provides an introductory explanation of the workings of the human body, with an effort to draw connections between the body systems and explain their interdependencies. A framework for the book is homeostasis and how the body maintains balance within each system. This is intended as a first introduction to physiology for a college-level course.

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