Part A

Cutting DNA

1" ^^ Wear safety goggles, gloves, and

2. Fill an ice bucket with ice. Obtain one each of the following microtubes: A—Uncut DNA, B—Hind\\\,

C—BamH\, D—EcoR\, and E—Unknown. Microtubes B-D contain 1 pL of the indicated restriction enzyme. Place all microtubes in the ice. Restriction enzymes MUST be kept on ice until step 6.

3. With a permanent marker, write the initials for everyone in your group on the top of microtubes A-E.

4. CAUTION If you get a chemical on your ^^ skin or clothing, wash it off at the sink while calling to your teacher. Set a micropipetter to 1 pL, and put a tip on the end of the micropipetter, as shown in (a) below. Using a new tip for each microtube, add 1 pL of the corresponding 10x restriction buffer to each of microtubes B-D. Place the buffer on the side of the tube. Do not touch the micropipetter tips to the solutions in the microtubes.

Sirens Sleep Solution

Sirens Sleep Solution

Discover How To Sleep In Peace And Harmony In A World Full Of Uncertainty And Dramatically Improve Your Quality Of Life Today! Finally You Can Fully Equip Yourself With These “Must Have” Tools For Achieving Peace And Calmness And Live A Life Of Comfort That You Deserve!

Get My Free Ebook

Post a comment