Materials

protective clothing

water bath (37°C)

ice, crushed

gel-casting tray

ice bucket

gel comb, 6-well

microtube rack

hot-water bath (65°C)

microtube A—Uncut DNA

agarose (0.8%)

microtube B—Hind\\\

hot mitt

microtube C—BamH\

zipper-lock plastic bag

microtube D—Eco R\

graduated cylinder

microtube E—Unknown

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