Materials

■ chromosome spread

■ human karyotyping form

■ photomicrograph of chromosomes

■ transparent tape

Background

1. Define and describe a human karyotype.

2. Compare the two procedures used to obtain the cells needed for preparing a fetal karyotype.

3. List four types of chromosomal mutations that can be identified by preparing a fetal karyotype.

4. Name the disorder that occurs in people who have an extra copy of chromosome 21.

Procedure

1. Obtain a photomicrograph and note the letter identifying which person the cells were taken from.

2. ^fck CAUTION Always cut in a direction away

▼^from your face and body. Carefully cut apart the chromosomes on each photomicrograph. Be sure to leave a slight margin around each chromosome.

3. Arrange the chromosomes in homologous pairs. The members of each pair will be the same length and will have their centromeres located in the same area. Use the ruler to measure the length of the chromosome and the position of the centromere. The banding patterns of homologous chromosomes will be similar and may also help you pair the chromosomes.

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