Info

CAUTION Handle the slide and coverslip

carefully. Glass slides break easily, and the sharp edges can cut you. Prepare a wet mount by placing part of a pink nodule on a microscope slide, adding a drop of water, and covering the slide with a coverslip.

13. Place the slide on a flat surface. Gently press down on the slide with your thumb. Use enough pressure to squash the nodule. Make sure that the coverslip does not slide.

14. Examine the slide under a microscope. Draw and label your observations in your lab report. Note the power of magnification used.

15. Compare your wet mount preparation with the prepared slide of Rhizobium bacteria and the photograph on the right. Cells with active Rhizobium bacteria should look like the reddish cells in the photograph at right.

16. ^jk Clean up your materials, and wash your hands before leaving the lab.

Analysis and Conclusions

1. Which plant had the most nodules?

2. How many nodules were found on the radish plants?

3. How do legumes become inoculated with bacteria in nature?

4. What kind of relationship exists between the legume plant and Rhizobium bacteria? How does this relationship benefit the legume plant? How does this relationship benefit the bacteria?

5. If you were to try to grow legumes without root nodules to use as experimental controls, why should you plant the seeds in sterile soil?

Further Inquiry

Perform the experiment by using beans with and without

Rhizobium bacteria. Count the number of leaves on each plant, and measure the mass of each whole plant as well as the masses of roots, stems, and leaves separately.

Predict which part of the plant will differ the most.

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