3. After 30 minutes have passed, attach screw clamps to each spot that you marked on the tubing. While your partner holds the corks firmly in place, tighten the middle clamp first, and then tighten the outer clamps.

4. Immediately pour the contents of each section of tubing into the test tube labeled with the corresponding number.

5. CAUTION Put on safety goggles before ^^ ^^ handling methyl cellulose. If you get methyl cellulose in your eyes, immediately flush it out at the eyewash station while calling to your teacher. Stopper test tube 1, and invert it gently to distribute the shrimp. Use a pipet to draw a 1 mL sample of shrimp culture and transfer the culture to a Petri dish. Add a few drops of methyl cellulose to the Petri dish to slow down the shrimp. Count the live shrimp, and record the count in your lab report.

6. Dispose of the shrimp as your teacher directs. ^^ Repeat step 5 four more times for a total of five counts from test tube 1.

7. Calculate the average number of shrimp in test tube 1, and record the result in the data table you made in your lab report.

8. Repeat steps 5-7 for the contents of each of the remaining test tubes.

,Clean up your materials, and wash your hands before leaving the lab.

10. In your lab report, make a histogram showing the number of shrimp in each section of tubing. Identify each section with the amount of screen.

Analysis and Conclusions

1. Describe the differences between the histogram of the control group and the histogram of the experimental group.

2. Why was a control (Part B) necessary?

3. How did the brine shrimp react to differences in light? Justify your conclusion.

Further Inquiry

Design an experiment to test the reaction of brine shrimp to a gradient of heat.

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