## Info

^ CAUTION Always wear safety goggles and a lab apron to protect your eyes and clothing. Put on safety goggles, a lab apron, and disposable gloves. Do not touch or taste any chemicals. Carry out the experiment you designed. Record your observations in your data table.

4. ^^ CAUTION Know the location of the emer-^^ gency shower and eyewash station and how to use them. If you get a chemical on your skin or clothing, wash it off at the sink while calling to the teacher. Notify the teacher of a spill. Spills should be cleaned up promptly, according to your teacher's directions.

5. CAUTION Glassware is fragile. Notify the ^^ teacher of broken glass or cuts. Do not clean up broken glass or spills with broken glass unless the teacher tells you to do so.

6. /irk Clean up your work area and all lab equipment. ^^ Return lab equipment to its proper place. Dispose of solutions, broken glass, and agar in the designated waste containers. Do not pour chemicals down the drain or put lab materials in the trash unless your teacher tells you to do so. Wash your hands thoroughly before you leave the lab and after you finish all work.

Analysis and Conclusions

1. Describe any changes in the appearance of the cubes.

2. Make a graph using your group's data. Plot "Diffusion distance (mm)" on the vertical axis. Use graph paper to plot "Surface area-to-volume ratio" on the horizontal axis.

3. Using the graph you made in item 2, make a statement about the relationship between the surface area-to-volume ratio and the distance a substance diffuses.

4. Make a graph using your group's data. Use graph paper to plot "Rate of diffusion (mm/min)" (distance vinegar moved time) on the vertical axis. Plot "Surface area-to-volume ratio" on the horizontal axis.

5. Using the graph you made in item 4, make a statement about the relationship between the surface area-to-volume ratio and the rate of diffusion of a substance.

6. In what ways do your agar models simplify or fail to simulate the features of real cells?

7. Calculate the surface area and volume of a cube with a side length of 5 cm. Calculate the surface area and volume of a cube with a side length of 10 cm. Determine the surface area-to-volume ratio of each of these cubes. Which cube has the greater surface area-to-volume ratio?

8. How does the size of a cell affect the diffusion of substances into the cell?

### Further Inquiry

Write a new question about cell size and diffusion that also explores hypotonic and hypertonic solutions that could be explored with another investigation.

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