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(b) CASTING TRAY

Reset the micropipetter to 8 pL. Using the micropipetter and a new tip for each microtube, add 8 pL of the Lambda virus DNA to the side of each of microtubes B-D. Gently flick the bottom of each microtube with your finger to mix the solutions. Do not shake the microtubes! Reset the micropipetter to 10 pL, and add 10 pL of Lambda DNA to microtube A.

Place all of the microtubes into a 37°C water bath. After 50-60 minutes, remove the microtubes from the water bath, and immediately put them into a freezer. If the class period ends before 50 minutes have passed, your teacher will give you further directions. While the restriction enzymes are working, go to Part B.

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