To investigate the mechanism of fibrosis progression in chronic pancreatitis, we studied the process of pancreatic fibrosis in male dogs (approximately 12 kg body weight) after oral administration of DL-ethionine (ethionine) as methionine analogue.
Group 1: Ethionine at a dose of 130mg/kg/day was given orally for 7 days. Dogs were sacrificed 1, 2, 3, 7 and 30 days after the last feeding.
Group 2: Ethionine at doses of 10, 50 and 100mg/kg was given once or twice a week for 10 or 20 weeks. Dogs were sacrificed 7 days after the last feeding.
Group 3: Ethionine at a dose of 75 mg/kg was given once a week for 15 weeks. Dogs were sacrificed 2, 3, 4 and 26 weeks after the last feeding.
Pancreas tissues were fixed in glutaraldehyde (2%) phosphate buffer (0.1 m, pH 7.4) at 4°C for 2 h and subjected to electron microscopic analysis. Other pancreas tissues were frozen for immunohistochemistry and fixed in formaldehyde (10%) for histochemistry and immunohistochemistry.
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