12.6 NOE Interactions Between One Residue and the Next Residue in the Sequence, 577

12.7 Sequence-Specific Assignment Using Homonuclear 2D Spectra, 580

12.8 Medium and Long-Range NOE Correlations, 586

12.9 Calculation of 3D Structure Using NMR Restraints, 590

12.10 15N-Labeling and 3D NMR, 596

12.11 Three-Dimensional NMR Pulse Sequences: 3D HSQC-TOCSY and 3D TOCSY-HSQC, 601

12.12 Triple-Resonance NMR on Doubly-Labeled (15N, 13C) Proteins, 610

12.13 New Techniques for Protein NMR: Residual Dipolar Couplings and Transverse Relaxation Optimized Spectroscopy (TROSY), 621

Appendix A: A Pictorial Key to NMR Spin States

Appendix B: A Survey of Two-Dimensional NMR Experiments


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