Sda

Methicillin Resistant Staphylococcus aureus

Real-time PCR

Mycobacterium tuberculosis

TMA, PCR

*See Chapters 7 and 9 for detailed description of these methods.

*See Chapters 7 and 9 for detailed description of these methods.

Manufacturers of these commercial reagents provide quality assurance requirements including controls and assay limitations. Each system must be validated on the type of specimen used for clinical testing, including serum, plasma, cerebrospinal and other body fluids, tissue, cultured cells, and organisms. In addition to the commercial reagent sets, many professionals working in clinical laboratories have been developing laboratory protocols (home-brew PCR) for most of the testing that they perform. Primers are designed based on sequence information that has been published; the reagents are bought separately, and the procedures are developed and optimized within the individual laboratory.

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