precipitation (ethanol)

Cells in suspension
■ Figure 4-6 Isolation of RNA on a solid matrix.

inside microfuge tubes (spin columns) are widely used for routine nucleic acid isolation from all types of specimens. The eluted RNA is usually of sufficient concentration and purity for direct use in most applications.

Generally, 1 million eukaryotic cells or 10-50 mg of tissue will yield about 10 ^g of RNA. The yield of RNA from biological fluids will depend on the concentration of microorganisms or other target molecules present in the specimen (Table 4.2).

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