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^Specimen handling especially effects RNA yield. Isolation of polyA RNA will result in much lower yields. See text. tRNA yield will depend on WBC count.

¿RNA yield will depend on type of cells and the conditions of cell culture.

§Liver, spleen, and heart tissues yield more RNA than brain, lung, ovary, kidney, or thymus tissues.

Isolation of RNA from fixed tissue is especially affected by the type of fixative used and the age and the preliminary handling of the original specimen.

^Different bacterial types and fungi will yield more or less RNA.

^Specimen handling especially effects RNA yield. Isolation of polyA RNA will result in much lower yields. See text. tRNA yield will depend on WBC count.

¿RNA yield will depend on type of cells and the conditions of cell culture.

§Liver, spleen, and heart tissues yield more RNA than brain, lung, ovary, kidney, or thymus tissues.

Isolation of RNA from fixed tissue is especially affected by the type of fixative used and the age and the preliminary handling of the original specimen.

^Different bacterial types and fungi will yield more or less RNA.

mRNA

AAAAAAAAA 3'

3'TTTTTTTTT 5'

Bead or column ■ Figure 4-7 Oligo polythymine columns or beads bind the polyA tail of mRNA. The oligo can be poly uracil. Peptide nucleic acid dU or dT can also be used.

Measurement of Nucleic Acid Quality and Quantity

Laboratory analysis of nucleic acids produces variable results, depending on the quality and quantity of input material. This is an important consideration in the clinical laboratory, as test results must be accurately interpreted with respect to disease pathology. Consistent results require that run-to-run variation be minimized. Fortunately, measurement of the quality and quantity of DNA and RNA is straightforward.

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