■ Agarose gel electrophoresis of SSP-PCR amplicons. Extension of a JAK2 V617F-specific primer produces a 270-bp band in addition to the normal 500- and 230-bp bands. Lane 1, molecular weight markers; lane 2, patient specimen; lane 3, V617F mutant control; lane 4, normal control; lane 5, reagent blank.

In the CBC, white blood cell and neutrophil counts were normal. Iron stores were also in normal range. A blood sample was submitted to the molecular pathology laboratory for JAK2 V617F mutation analysis. The results are shown below:



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