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■ Figure 12-5 PFGE results of seven test strains (top panel). The fragment shifts are marked in the middle panel. Strains 1, 3, and 5 are the same as the outbreak strain (O). Strains 2 and 6 have apparently one genetic difference, resulting in two fragment differences. Strain 4 has seven fragment differences, indicating more than six genetic differences and is not related to the outbreak. PFGE of coagulase-negative Staphylococcus (bottom). Lanes 1 and 7, molecular weight markers; lanes 2 and 3 are two isolates that are same; lanes 4, 5, and 6 are three isolates that are the same but different from the isolates in lanes 2 and 3. (Courtesy of Mary Hayden, MD, Rush Medical Laboratories, Rush University Medical Center, Chicago, IL.)

■ Figure 12-5 PFGE results of seven test strains (top panel). The fragment shifts are marked in the middle panel. Strains 1, 3, and 5 are the same as the outbreak strain (O). Strains 2 and 6 have apparently one genetic difference, resulting in two fragment differences. Strain 4 has seven fragment differences, indicating more than six genetic differences and is not related to the outbreak. PFGE of coagulase-negative Staphylococcus (bottom). Lanes 1 and 7, molecular weight markers; lanes 2 and 3 are two isolates that are same; lanes 4, 5, and 6 are three isolates that are the same but different from the isolates in lanes 2 and 3. (Courtesy of Mary Hayden, MD, Rush Medical Laboratories, Rush University Medical Center, Chicago, IL.)

Table 12.9 Criteria for PFGE Pattern Interpretation

Category

Genetic Differences*

Fragment Differences*

Epidemiological Interpretation

Indistinguishable

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