Chapter

What actions should be taken in the following situations?

1. An unlabeled collection tube with a requisition for a factor V Leiden test is received in the laboratory.

Notify the supervisor and reject the specimen.

2. After PCR, the amplification control has failed to yield a product.

Check the original DNA or RNA preparation. If it is adequate, repeat the amplification. If not, reisolate the nucleic acid.

3. An isolated DNA sample is to be stored for at least 6 months.

Store at -70°C in a tightly sealed tube.

4. A bone marrow specimen arrives at the end of a shift and will not be processed for the Bcl2 translocation until the next day.

Place the specimen in the refrigerator.

5. The temperature of a refrigerator set at 8°C (±2°C) reads 14°C.

Recheck the temperature after a few hours. If it does not return to range, notify the supervisor.

6. A PCR test for the BCR/ABL translocation was negative for the patient sample and for the sensitivity control.

Repeat the PCR with the addition of a new sensitivity control.

7. A fragile X test result has been properly reviewed and reported.

File the test results, documents, and associated autoradiographs together in the laboratory archives.

8. A bottle of reagent alcohol with a 3 in the red diamond on its label is to be stored.

Place the alcohol bottle in a safety storage cabinet for flammable liquids.

9. The expiration date on a reagent has passed. Discard the reagent. If it can be used for research or other nonclinical purposes, label and store it in a separate area away from patient testing reagents.

10. Test results are to be faxed to the ordering physician. Fax the results with a cover sheet containing the proper disclaimer.

Index

Numbers followed by b represent boxes, numbers followed by f represent figures, and numbers followed by t represent tables.

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