Gels in cassette systems and gel strip systems can be loaded without loading buffer because the wells are "dry," precluding the need for density gradients. As these systems also have automatic shut-off at the end of the run, tracking dye is usually not necessary, although some of these systems have a tracking dye built into the gel and/or buffer.
Following are the status of samples during and after electrophoresis is accomplished using dyes that specifically associate with nucleic acid. The agents used most frequently for this application are fluorescent dyes and silver stain.
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