Advanced Concepts

Newer models of ultraviolet spectrophotometers dedicated to nucleic acid analysis can be programmed to do the calculations described. The operator must enter the type of nucleic acid, the dilution factor, and the desired conversion factor. The instrument will automatically read the sample at both wavelengths and do the required calculations, giving a reading of concentration in ^g/mL and a 260 nm/280 nm ratio.

analysis are not affected by contaminating RNA, in which case the DNA is still suitable for use. If, however, RNA may interfere or react with DNA detection components, RNase should be used to remove the contaminating RNA. Because it is difficult to detect contaminating DNA in RNA preparations, RNA should be treated with RNase-free DNase where DNA contamination may interfere.

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