Methods In Enzymology Vol 254

Copyright 1995 by Academic Press, Inc. All rights of reproduction in any form reserved. oncogenic changes it is necessary to identify the controls that operate during normal hematopoiesis and then determine how these are disturbed in the various disease states. Our current understanding of how normal and abnormal hematopoiesis is controlled stems mainly from work on murine and human hematopoiesis. However, it has been difficult to reconstruct the different oncogenic alterations causing...

Graft Chamber Mice Skin

Chamber Grafting Skin

Continuous human cell line, contains Adl2-SV40. h Papilloma cell lines, contain activated c-rasHa. ' Phenotype dependent on high v-rasHa expression. '' Cultured epithelial sheet implanted intact between dorsal musculature and skin. Skin reconstitution or grafting is a more widely used method since it has the advantage of allowing the study of cells at intermediate stages of premalignant progression. In some studies the graft site on the host is prepared by inserting a ground glass disk (26 x 3...

Contributors to Volume 254

Article numbers are in parentheses following the names of contributors. Affiliations listed are current. Stuart A. Aaronson (13), Derald H. Ruttenberg Cancer Center, Mount Sinai Medical Center, New York, New York 10029 Victor Adler (37), American Health Foundation, Valhalla, New York 10595 Leila Alland (36), Albert Einstein College of Medicine, Bronx, New York 11046 Christopher P. Austin (25), Department of Genetics, Harvard University Medical School, Boston, Massachusetts 02115 Lidia Averboukh...

[1 Isolation and Utilization of Epidermal Keratinocytes for Oncogene Research

Glick, Tamar Tennenbaum, Wendy C. Weinberg, and Stuart H. Yuspa Keratinocytes have been widely used as target cells for testing the activity of oncogenes in epithelial neoplasia. Many experimental studies have utilized cultured mouse skin keratinocytes, where in vitro results can be analyzed in the context of a substantial experience in carcinogen-induced mouse skin tumors.1 More recent experiments have employed keratinocytes derived from human skin, oral cavity,...

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Filter Through for 30 Minutes at 37 Nylon Gauze Fig. 1. Protocol for isolating primary keratinocytes from newborn mouse skin. See text for additional details. neoplastic progression e.g., fos, mutant p53, ras profoundly influence keratinocyte growth control.20-22,27 Newborn mouse epidermis yields a large number of cells 5-10 X 106 epidermis , with a 30-40 plating efficiency. Mice are sacrificed by C02 narcosis 1-4 days postpartum prior to the appearance of hair , soaked in Betadine for 5 min,...