Inoculum Expansion and Production Steps

Very limited information is available in the public domain regarding the general design and sizing of an upstream processing line for pilot- or large-scale production. Chu and Robinson (2001) present an overview of the technologies (bioreactor type and mode, medium type) for the various commercial products recently approved (recombinant proteins, vaccines, diagnostics and tissue culture), but few details on the scale of the cultivation vessels are actually available. In the following, typical...

Expression Vectors 5221 Plasmid vectors

Cho Protein Production

A wide selection of plasmid-based expression vectors is now available. For many studies of gene expression, small quantities of protein are sufficient and can be obtained using transient assay systems. When larger amounts of protein are required, it is necessary to identify clonal cell lines in which the vector sequences are retained during cellular proliferation 'stable' cell lines . This can be achieved either by episomal plasmid replication or by the integration of the vector into the host...

Equipment and Scaleup

Column Packing

The availability of hardware at the required scale, or the complexity of its deployment, may influence which techniques are used and how they are implemented. Large-scale engineering, for example of flow distributors, can be a critical issue. The difficulties of large-scale operation can be balanced against the cost of running multiple cycles at a Figure 18.3 Pilot-scale columns used in Phase II clinical manufacture. A 30-litre size exclusion column and a 2-litre ion exchange column used for...