Principles of Bacterial Growth

Bacteria generally multiply by the process of binary fission. After a bacterial cell has increased in size and doubled all of its parts, it divides (figure 4.3). One cell divides into two, those two divide to become four, those four become eight, and so on. In other words, the increase in cell numbers is exponential. Because it is

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4.2 Principles of Bacterial Growth 85

(1) Loop is sterilized

(1) Loop is sterilized

Streak Plate Method

(6) Loop is sterilized

(8) Isolated colonies develop after incubation

Figure 4.2 The Streak-Plate Method A sterilized inoculating loop (1) is dipped into a culture (2) and is then lightly drawn several times across an agar plate (3).The loop is sterilized again (4), and a new series of streaks is made at an angle to the first set (5).The loop is sterilized a final time (6), and another set of parallel streaks is made (7).The successive streaks dilute the concentration of cells. By the third set of streaks, cells should be separated enough so that isolated colonies develop after incubation (8).

(6) Loop is sterilized

4.2 Principles of Bacterial Growth 85

Figure 4.2 The Streak-Plate Method A sterilized inoculating loop (1) is dipped into a culture (2) and is then lightly drawn several times across an agar plate (3).The loop is sterilized again (4), and a new series of streaks is made at an angle to the first set (5).The loop is sterilized a final time (6), and another set of parallel streaks is made (7).The successive streaks dilute the concentration of cells. By the third set of streaks, cells should be separated enough so that isolated colonies develop after incubation (8).

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    What are the principal of microbial growth?
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