1. Explain what the term semiconservative means with respect to DNA replication.
2. How can E. coli have a generation time of only 20 minutes when it takes 40 minutes to replicate its chromosome?
3. What is the function of primase in DNA replication? Why is this enzyme necessary?
4. What is polycistronic mRNA?
5. Explain why knowing the orientation of a promoter is critical when determining the amino acid sequence of an encoded protein.
6. What is characteristic about the nucleotide sequence of a transcription terminator?
7. What happens to a polypeptide that has a signal sequence?
8. Compare and contrast regulation by a repressor and an activator.
9. Explain how bacteria sense the density of cells in their own population.
10. Explain why it is sometimes difficult to locate genomic regions that encode a protein.
Multiple Choice Questions
1. All of the following are involved in transcription, except A. polymerase. B. primer. C. promoter. D. sigma factor. E. uracil.
2. All of the following are involved in DNA replication, except A. elongation factors. B. gyrase. C. polymerase. D. primase. E. primer.
3. All of the following are directly involved in translation, except A. promoter. B. ribosome. C. start codon. D. stop codon. E. tRNA.
4. Using the DNA strand depicted here as a template, what will be the sequence of the RNA transcript?
5' GCGTTAACGTAGGC 3'
A ribosome binds to the following mRNA at the site indicated by the dark box. What are the first three amino acids that will be incorporated into the resulting polypeptide?
3' CGCAATTGCATCCG 5'
A. 5' GCGUUAACGUAGGC 3'
B. 5' CGGAUGCAAUUGCG 3'
C. 5' CGCAAUUGCAUCCG 3'
D. 5' GCCUACGUUAACGC 3'
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