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significance of this pathway is that it differentiates certain members of the family Enterobacteriaceae. The Voges-Proskauer test is used to detect acetoin, distinguishing bacteria that use this pathway, such as Klebsiella and Enterobacter, from those that do not, such as E. coli (see table 10.4). ■ Voges-Proskauer test, p. 252

■ Mixed acids. These are produced in a multistep branching pathway, generating a variety of different fermentation end products including lactic acid, succinic acid (the ionized form is succinate), ethanol, acetic acid (the ionized form is acetate), CO2, and gases. The primary significance of this pathway is that it differentiates certain members of the family Enterobacteriaceae. The methyl-red test is used to detect end products of the pathway, distinguishing bacteria using this pathway, such as E. coli, from those that do not, such as Klebsiella and Enterobacter (see table 10.4). ■ methyl-red test, p. 252

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