The dideoxy chain termination method is a widely used sequencing technique.

■ Describe the chemical difference between a ddNTP and a dNTP.

■ How does a ddNTP terminate DNA synthesis?

■ What would happen in the sequencing reaction if the relative concentration of a dideoxynucleotide were increased?

Figure 9.21 An Autoradiograph of a Sequencing Gel Each dark band represents a specific-sized fragment.The sequence of the newly synthesized strand can be determined by "reading'' the gel from the bottom up.

Bacterial Vaginosis Facts

Bacterial Vaginosis Facts

This fact sheet is designed to provide you with information on Bacterial Vaginosis. Bacterial vaginosis is an abnormal vaginal condition that is characterized by vaginal discharge and results from an overgrowth of atypical bacteria in the vagina.

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