The dideoxy chain termination method is a widely used sequencing technique.

■ Describe the chemical difference between a ddNTP and a dNTP.

■ How does a ddNTP terminate DNA synthesis?

■ What would happen in the sequencing reaction if the relative concentration of a dideoxynucleotide were increased?

Figure 9.21 An Autoradiograph of a Sequencing Gel Each dark band represents a specific-sized fragment.The sequence of the newly synthesized strand can be determined by "reading'' the gel from the bottom up.

Was this article helpful?

0 0
How To Cure Yeast Infection

How To Cure Yeast Infection

Now if this is what you want, you’ve made a great decision to get and read this book. “How To Cure Yeast Infection” is a practical book that will open your eyes to the facts about yeast infection and educate you on how you can calmly test (diagnose) and treat yeast infection at home.

Get My Free Ebook

Post a comment