Microcheck

The dideoxy chain termination method is a widely used sequencing technique.

■ Describe the chemical difference between a ddNTP and a dNTP.

■ How does a ddNTP terminate DNA synthesis?

■ What would happen in the sequencing reaction if the relative concentration of a dideoxynucleotide were increased?

Figure 9.21 An Autoradiograph of a Sequencing Gel Each dark band represents a specific-sized fragment.The sequence of the newly synthesized strand can be determined by "reading'' the gel from the bottom up.

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