A colony blot detects specific sequences in DNA released from lysed cells that have been replica-plated onto a solid support. A Southern blot requires multiple steps, including digestion and gel electrophoresis of DNA, transferring the DNA to a solid membrane support, and detecting specific DNA sequences. Sample preparation is a critical aspect of FISH. Sophisticated techniques are used to construct nucleotide microarrays.
■ Describe two ways to obtain a DNA probe.
■ What is the purpose of gel electrophoresis?
■ In what situation is it important to distinguish between different bacterial strains?
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