Nucleic acid probes detect specific nucleotide sequences in a sample. Colony blots are used to identify colonies that contain a given sequence of DNA. Southern blots are used to determine the size of the restriction fragments that contain a sequence of interest and to detect subtle variations in nucleotide sequences that occur in different strains of a given species. Fluorescence in situ hybridization is used to observe cells in intact specimens that contain a given sequence. Nucleotide array technology enables a researcher to

9.2 Applications of Nucleic Acid Hybridization 227

Strain A DNA

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