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When grown in a closed system, a population of bacteria goes through five distinct phases: lag, log, stationary, death, and prolonged decline. Cells within a colony may be in any one of the growth phases, depending on their relative location.

■ Explain the difference between the two stages of the exponential phase.

■ Describe how a chemostat keeps a culture in a continuous stage of growth.

■ Why would bacteria be more susceptible to antibiotics during the log phase?

Figure 4.19 Dynamic Population Changes in the Phase of Prolonged Decline Many members of the population are dying and releasing their nutrients, while a few "fitted" cells are actively multiplying.

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