Culture media may be either complex or chemically defined. Media may contain additional ingredients that make them selective or differential. Appropriate atmospheric conditions must be provided to isolate microaerophiles and anaerobes. An enrichment culture increases the relative concentration of an organism growing in a broth.

■ Distinguish between a selective medium and a differential medium.

■ Describe two methods used to create anaerobic conditions.

■ Would bacteria that cannot utilize lactose be able to grow on MacConkey agar?

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