Only an estimated 1% of prokaryotes can be cultured in the laboratory. Agar is used to solidify nutrient-containing broth. The streak-plate method is used to obtain a pure culture.
■ What properties of agar make it ideal for use in bacteriological media?
■ How does the streak-plate method separate individual cells?
■ What might be a reason that medically significant bacteria can be grown in pure culture more often than environmental organisms?
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