Microcheck 102

The size, shape, and staining characteristics of a microorganism, all of which can be viewed using a microscope, yield important clues to its identity. Conclusive identification generally requires a battery of biochemical tests that assay for compounds indicating the presence of specific biochemical pathways. The proteins and polysaccharides that make up a bacterium

Oxidase test

Figure 10.6 Commercial Modifications of Traditional Biochemical Tests These methods are less laborintensive than traditional tests. (a) An API™ test strip. Each of the cupules contains dehydrated medium similar in formulation to the traditional tests. A liquid suspension of the isolated test bacterium is added to each compartment; after incubation, the results are read manually.The upper panel shows positive results, and the lower panel shows negative results. (b) An Enterotube.™ Each compartment contains a different type of medium. One end of a metal rod that runs through the tube is used to touch a bacterial colony.When the rod is withdrawn it inoculates each of the compartments. After incubation, the results are read manually.

Figure 10.6 Commercial Modifications of Traditional Biochemical Tests These methods are less laborintensive than traditional tests. (a) An API™ test strip. Each of the cupules contains dehydrated medium similar in formulation to the traditional tests. A liquid suspension of the isolated test bacterium is added to each compartment; after incubation, the results are read manually.The upper panel shows positive results, and the lower panel shows negative results. (b) An Enterotube.™ Each compartment contains a different type of medium. One end of a metal rod that runs through the tube is used to touch a bacterial colony.When the rod is withdrawn it inoculates each of the compartments. After incubation, the results are read manually.

Nester-Anderson-Roberts: I II. The Microbial World I 10. Identification and I I © The McGraw-Hill

Microbiology, A Human Classification of Companies, 2003

Perspective, Fourth Edition Prokaryotes

254 Chapter 10 Identification and Classification of Prokaryotes

P. cepacia

P. aeruginosa

P. cepacia

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