Microbiology laboratories routinely work with microbial cultures and consequently must use rigorous methods of controlling microorganisms. To work with pure cultures, all media and instruments that contact the culture must first be rendered sterile to avoid contaminating the culture with environmental bacteria. All materials used to grow microorganisms must again be treated
112 Chapter 5 Control of Microbial Growth before disposal to avoid contamination of workers and the environment. The use of specific methods to exclude contaminating microorganisms from an environment is called aseptic technique. Although all microbiology laboratory personnel must use these prudent measures, those who work with known disease-causing microbes must be even more diligent.
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