Membrane Filtration

Membrane filtration is used when the numbers of organisms in a sample are relatively low, as might occur in dilute environments such as natural waters. This method concentrates the bacteria by filtration before they are plated. A known volume of liquid is passed through a sterile membrane filter, which has a pore size that retains bacteria (figure 4.14). The filter is subse

(a)

Figure 4.14 Membrane Filtration This technique concentrates bacteria before they are plated. (a) A known volume of liquid is passed through a sterile membrane filter, which has a pore size that retains bacteria. (b)The filter is then placed on an appropriate agar medium and incubated.The number of colonies that grow on the filter indicates the number of bacteria that were in the volume filtered.

Figure 4.14 Membrane Filtration This technique concentrates bacteria before they are plated. (a) A known volume of liquid is passed through a sterile membrane filter, which has a pore size that retains bacteria. (b)The filter is then placed on an appropriate agar medium and incubated.The number of colonies that grow on the filter indicates the number of bacteria that were in the volume filtered.

quently placed on an appropriate agar medium and then incubated. The number of colonies that grow on the filter indicates the number of bacteria that were in the volume filtered.

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