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Chapter 8 Bacterial Genetics

Phage

Phage DNA enters the cell.

Bacterial host #1

Empty phage coat remains on outside of bacterium.

Phage DNA enters the cell.

Bacterial host #1

When a phage infects a host cell, it may cause the degradation of host DNA into small fragments.

Bacterial DNA

Bacterial DNA

The phage carrying the bacterial DNA infects another cell, transferring the bacterial DNA into the new cell.

Replaced host DNA

Replaced host DNA

Figure 8.16 Transduction (Generalized) Any piece of the chromosomal DNA of the donor cell can be transferred in this process. All of the DNA molecules, of the bacterial virus and of the bacteria, are double-stranded.

When a phage infects a host cell, it may cause the degradation of host DNA into small fragments.

Phage coat proteins are synthesized and phage DNA is replicated.

During maturation of the virus particles, a few phage heads may envelop fragments of bacterial DNA instead of phage DNA. Only bacterial DNA is present in the transducing virions.

The phage carrying the bacterial DNA infects another cell, transferring the bacterial DNA into the new cell.

Bacterial host #2

When this bacterial DNA is introduced into a new host cell, it can become integrated into the bacterial chromosome, thereby transferring several bacterial genes at one time. Only genes that are located close together will be transduced together.

Bacteria multiply with new genetic material.

Figure 8.16 Transduction (Generalized) Any piece of the chromosomal DNA of the donor cell can be transferred in this process. All of the DNA molecules, of the bacterial virus and of the bacteria, are double-stranded.

cells when the virus invades these cells. This bacterial DNA is then integrated into the recipient cell DNA. This is the process of transduction.

■ What effect would the addition of deoxyribonuclease to the cells have on this process?

■ If gene 1 is on the chromosome and gene 2 is on a plasmid, which one would be transduced at a higher frequency? Explain.

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