Eukaryotic DNA

Precursor mRNA

mRNA (within eukaryotic cells)

f mRNA (isolated)

Single strand of DNA

Double-stranded DNA

Transcription of eukaryotic DNA generates precursor mRNA

Figure 9.13 Making cDNA from Eukaryotic mRNA In order for eukaryotic genes to be expressed by a prokaryotic cell, a copy of DNA without introns must be cloned.The cDNA encodes the same protein as the original DNA but lacks introns

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